In medical clinic, serum protein electrophoresis and immunofixation protein electrophoresis are both the most important indexes, which are the core of the detection system. However, a lot of people know little about it. In this paper, the safety net will explain: what is serum protein electrophoresis?
What is serum protein electrophoresis?
Serum protein is the protein in serum. So, what is serum? Put the extracted blood into a test tube without anticoagulant to make the blood coagulate. The supernatant is serum. Compared with plasma, the protein in serum is less fibrinogen, but mainly albumin and globulin.
Protein is a kind of amphoteric electrolyte, which has positive charge when it dissolves in acidic solution and negative charge when it dissolves in alkaline solution. If the serum protein is placed in the buffer solution of ph8.6, all kinds of proteins in the serum are negatively charged and move to the positive electrode in the electric field. Because of the different molecular sizes of various proteins and the amount of electric charge, the speed of moving in the electric field varies. In medicine, we use this property to divide the sequence of serum proteins on the electrophoresis membrane into albumin (a) and four kinds of globulins (represented by α 1, α 2, β, γ respectively). According to the results of electrophoresis, we can help diagnose diseases. This kind of inspection method is called serum protein electrophoresis.
The values of serum protein electrophoresis (filter paper method) were 55-61% for albumin, 4-5% for α 1 globulin, 6-9% for α 2 globulin, 9-12% for β globulin and 15-20% for γ globulin.
Since most of the proteins are synthesized by the liver, the changes of the quality and quantity of various proteins mainly reflect the physiological and pathological conditions of the liver, in addition to being related to immune diseases.
Warm tip: Fasting for 12 hours, take venous blood for test, and make sure fresh blood is available. Before the test, the doctor should be informed of the drugs taken and the recent physiological changes. If you want to know more about children’s home electric shock prevention, please go to this safety net to find relevant information.